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Overview of strategies developed for predicting alternate states with AlphaFold2 (AF2). As done in del Alamo et al.12 the Multiple Sequence Alignment (MSA) depth can be altered, some of the MSA positions can be masked as shown by Stein and McHaourab,13 and the MSA can be clustered as in the Kern and Ovchinnikov preprint paper.37 The provided set of templates can also be changed as done in some cases in del Alamo et al.12 and also in our recent publication.34
