Table 1.
PCR primer sequences used to amplify the genes of interest. Forward and reverse primers, melting temperatures (Tm), predicted product size and accession numbers are shown.
| Gene | Forward Sequence 5′-3′ Reverse Sequence 3′–5′ |
Tm (°C) | Product Size | Accession No. |
|---|---|---|---|---|
| ATF6 | GAGCTGTCTGTGTGATGATAGT CTAGGTTTCACTCTTCGGGATT |
59.88 59.90 |
94 | NM_001081304.1 |
| CD206 | AGTGATGGTTCTCCCGTTTC ACCTTTCAGCTCACCACAAT |
60.15 59.91 |
90 | NM_008625.2 |
| IRE1α (ERN1) | GAGACAAAGGAGAGTGTGTGAT TCAAGTAGTTCAGCTTGCTCTT |
60.05 59.81 |
87 | NM_023913.2 |
| TREM2 | CATCACTCTGAAGAACCTCCAA CTCCAGCATCTTGGTCATCTA |
60.10 59.46 |
137 | NM_031254.3 |
| PERK (EIF2AK3) |
CCTTGGTTTCATCTAGCCTCA ACTTGTAGGAAGATTCGAGCAG |
59.95 60.12 |
156 | NM_010121.3 |
| XBP1 | CAGAGAGTCAAACTAACGTGGT CAATGGCTGGATGAAAGCAG |
60.21 59.89 |
160 | NM_00127130.1 |
| IBA1 | GCTTTTGGACTGCTGAAGGC GTTTGGACGGCAGATCCTCA |
60.04 61.45 |
114 | NM_001361501.1 |
| IL-6 | CCCCAATTTCCAATGCTCTCC CGCACTAGGTTTGCCGAGTA |
59.24 60.11 |
141 | NM_031168.2 |
| DDIT3 | AGAAGGAAATGGAACGCACA CCAGCTGTGATGTGGGATAA |
60.16 60.01 |
135 | NM_008654.2 |
| ATF4 | CCTCAGACAGTGAACCCAAT AATGCTCTGGAGTGGAAGAC |
59.89 59.91 |
127 | NM_009716.3 |
| GADD34 | AGAAGGAAATGGAACGCACA CCAGCTGTGATGTGGGATAA |
60.16 60.01 |
135 | NM_008654.2 |
| S18 | CCCTGAGAAGTTCCAGCACA GGTGAGGTCGATGTCTGCTT |
59.60 59.75 |
145 | NM_011296.2 |