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. Author manuscript; available in PMC: 2023 Dec 22.
Published in final edited form as: J Med Chem. 2022 Dec 2;65(24):16510–16525. doi: 10.1021/acs.jmedchem.2c01380

Figure 4. Post antibiotic effect of the ACC inhibitors against E. coli ΔtolC.

Figure 4.

A) PAE of 15; B) PAE of 16; C) PAE of 17; D) PAE of 18; E) PAE of 19, F) PAE of 20. Cultures of E. coli ΔtolC (106 CFU/mL) were treated with 6 μM (8 μg/mL) PMBN and 0x, 8x, 16x, or 32x MIC of each inhibitor for 1 h followed by 1:1000-fold dilution into fresh cation-adjusted Mueller-Hinton (CaMH) media at 37 °C. Samples (100 μL) of the diluted cultures were then plated on Muller-Hinton agar plates every hour, and CFUs enumerated following incubation of the plates at 37 °C for 16 h. The experimental data points are the mean values from duplicate, independent measurements, and the error bars represent the standard deviation from the mean.