Table 1.
Design of experiments using the Box–Behnken method to optimize the niosomal formulation of Thymol.
| Run | Levels of Independent Variables | Dependent Variables | ||||
|---|---|---|---|---|---|---|
| Span 60 (mM) | Cholesterol (mM) |
Volume Hydration (mL) | Average Size (nm) | PDI | Entrapment Efficiency (EE) (%) | |
| 1 | 3 | 0.5 | 6 | 219 | 0.24 | 70 |
| 2 | 5 | 0.5 | 8 | 194 | 0.25 | 68 |
| 3 | 1 | 1.5 | 6 | 270 | 0.42 | 62 |
| 4 | 3 | 0.5 | 10 | 165 | 0.3 | 67 |
| 5 | 3 | 1.5 | 8 | 190 | 0.24 | 64 |
| 6 | 1 | 0.5 | 8 | 212 | 0.29 | 60 |
| 7 | 3 | 2.5 | 6 | 230 | 0.35 | 53 |
| 8 | 3 | 1.5 | 8 | 183 | 0.25 | 65 |
| 9 | 5 | 1.5 | 6 | 216 | 0.18 | 62 |
| 10 | 1 | 1.5 | 10 | 223 | 0.23 | 47 |
| 11 | 5 | 2.5 | 8 | 205 | 0.21 | 57 |
| 12 | 3 | 2.5 | 10 | 236 | 0.26 | 51 |
| 13 | 1 | 2.5 | 8 | 242 | 0.28 | 45 |
| 14 | 3 | 1.5 | 8 | 199 | 0.23 | 66 |
| 15 | 5 | 1.5 | 10 | 181 | 0.23 | 57 |