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. 2023 Jun 20;28(12):4880. doi: 10.3390/molecules28124880

Figure 5.

Figure 5

Confirmation of genetic transformation by PCR analysis of genomic DNA isolated from Rindera graeca shoots regenerated from roots. (a) RgTR17 line: M—molecular weight marker (GeneRulerTM 1 kb Plus DNA ladder); lane 1—virG; lane 2—rolB (423 bp); lane 3—rolC (626 bp); lane 4—Agrobacterium rhizogenes 15834 plasmid—virG (273 bp). (b) M—molecular weight marker GeneRulerTM 100 bp DNA ladder; lanes 1–6: RgAR shoot line; lanes 7–9: RgCR/NOA shoot line; lanes 10–12: RgTR7 shoot line: 10—virG, 11—rolB (423 bp), 12—rolC (626 bp); lanes 13–15: RgCR/NOA shoot line; lanes 16–18: A. rhizogenes 15834 plasmid: lane 16—virG (273 bp), lane 17—rolB (423 bp), and lane 18—rolC (626 bp).