Table 2.
Flask Cultures | |||||||||
Rosmarinic Acid | Lithospermic Acid B | ||||||||
Shoot line | Medium | ||||||||
DCR | DCR BAP | SH | SH BAP | DCR | DCR BAP | SH | SH BAP | ||
RgS | na | 12.7 ± 4.0 | na | na | na | 22.5 ± 5.6 a | na | na | |
RgAR | 14.3 ± 4.0 a | 11.9 ± 1.5 * | 19.2 ± 3.0 * | 17.6 ± 1.6 * | 15.3 ± 8.1 * | 49.3 ± 15.5 a,* | 20.8 ± 9.4 | 27.9 ± 2.2 a | |
RgCR/NOA | 12.9 ± 4.6 a,* | 14.7 ± 3.4 | 22.0 ± 4.8 * | 19.3 ± 2.3 * | 8.6 ± 1.1 * | 9.9 ± 0.5 a | 12.3 ± 2.6 * | 9.2 ± 0.8 a,* | |
RgTR7 | 24.6 ±5.3 a,* | 8.6 ± 1.4 * | 16.6 ± 2.4 * | 14.7 ± 1.0 * | 9.7 ± 1.2 * | 15.8 ± 11.9 a,* | 11.4 ± 2.2 a | 11.6 ± 1.4 a,* | |
RgTR17 | 15.3 ± 5.4 a,* | 10.0 ± 1.0 * | 21.7 ± 1.8 * | 16.7 ± 2.6 | 9.3 ± 3.5 | 9.4 ± 1.1 a | 15.3 ± 1.7 a,* | 11.0 ± 2.3 a,* | |
Root line | Medium | ||||||||
DCR | SH | DCR | SH | ||||||
RgAR | 14.4 ± 5.9 a,* | 1.2 ± 0.4 a,* | 0.3 ± 0.1 * | 8.5 ± 2.1 a,* | |||||
RgCR/NOA | 10.0 ± 2.5 * | 2.5 ± 1.4 a,* | 0.2 ± 0.03 * | 20.5 ± 5.5 a,* | |||||
RgTR7 | 7.4 ± 1.7 a | 2.6 ± 1.5 a,* | 0.3 ± 0.06 * | 23.1 ± 6.6 a,* | |||||
RgTR17 | 8.2 ± 1.2 a | 2.4 ± 0.9 a,* | 0.3 ± 0.06 * | 28.9 ± 7.7 a,* | |||||
Bioreactor cultures | |||||||||
Rosmarinic acid | Lithospermic Acid B | ||||||||
DCR | SH | DCR | SH | ||||||
Shoots regenerated from RgAR roots | na | 30.0 ± 3.2 a,b | na | 13.1 ± 1.7 a | |||||
RgAR roots | na | 5.6 ± 0.7 a,b | na | 0 | |||||
Shoots regenerated from RgTR17 roots | na | 11.6 ± 0.9 a,c | na | 3.9 ± 0.2 a | |||||
RgTR17 roots | 2.6 ± 0.2 a | 2.1 ± 0.1 a,c | 0.4 ± 0.01 | 0 | |||||
RgTR7 roots | 3.4 ± 0.3 a | na | 0 | na |
Data represent means ± SD. Differences within the group denoted with the same letter are statistically significant (p < 0.05); asterisks denote statistically significant differences between shoot/root lines cultivated in different media; na—not applicable. The concentration of BAP was 0.5 mg/L.