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. 2023 Jun 20;28(12):4880. doi: 10.3390/molecules28124880

Table 2.

Phenolic acid content (mg/g DW) in shoots and roots of Rindera graeca cultivated in vitro.

Flask Cultures
Rosmarinic Acid Lithospermic Acid B
Shoot line Medium
DCR DCR BAP SH SH BAP DCR DCR BAP SH SH BAP
RgS na 12.7 ± 4.0 na na na 22.5 ± 5.6 a na na
RgAR 14.3 ± 4.0 a 11.9 ± 1.5 * 19.2 ± 3.0 * 17.6 ± 1.6 * 15.3 ± 8.1 * 49.3 ± 15.5 a,* 20.8 ± 9.4 27.9 ± 2.2 a
RgCR/NOA 12.9 ± 4.6 a,* 14.7 ± 3.4 22.0 ± 4.8 * 19.3 ± 2.3 * 8.6 ± 1.1 * 9.9 ± 0.5 a 12.3 ± 2.6 * 9.2 ± 0.8 a,*
RgTR7 24.6 ±5.3 a,* 8.6 ± 1.4 * 16.6 ± 2.4 * 14.7 ± 1.0 * 9.7 ± 1.2 * 15.8 ± 11.9 a,* 11.4 ± 2.2 a 11.6 ± 1.4 a,*
RgTR17 15.3 ± 5.4 a,* 10.0 ± 1.0 * 21.7 ± 1.8 * 16.7 ± 2.6 9.3 ± 3.5 9.4 ± 1.1 a 15.3 ± 1.7 a,* 11.0 ± 2.3 a,*
Root line Medium
DCR SH DCR SH
RgAR 14.4 ± 5.9 a,* 1.2 ± 0.4 a,* 0.3 ± 0.1 * 8.5 ± 2.1 a,*
RgCR/NOA 10.0 ± 2.5 * 2.5 ± 1.4 a,* 0.2 ± 0.03 * 20.5 ± 5.5 a,*
RgTR7 7.4 ± 1.7 a 2.6 ± 1.5 a,* 0.3 ± 0.06 * 23.1 ± 6.6 a,*
RgTR17 8.2 ± 1.2 a 2.4 ± 0.9 a,* 0.3 ± 0.06 * 28.9 ± 7.7 a,*
Bioreactor cultures
Rosmarinic acid Lithospermic Acid B
DCR SH DCR SH
Shoots regenerated from RgAR roots na 30.0 ± 3.2 a,b na 13.1 ± 1.7 a
RgAR roots na 5.6 ± 0.7 a,b na 0
Shoots regenerated from RgTR17 roots na 11.6 ± 0.9 a,c na 3.9 ± 0.2 a
RgTR17 roots 2.6 ± 0.2 a 2.1 ± 0.1 a,c 0.4 ± 0.01 0
RgTR7 roots 3.4 ± 0.3 a na 0 na

Data represent means ± SD. Differences within the group denoted with the same letter are statistically significant (p < 0.05); asterisks denote statistically significant differences between shoot/root lines cultivated in different media; na—not applicable. The concentration of BAP was 0.5 mg/L.