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. 2023 May 31;15(6):1302. doi: 10.3390/v15061302

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© 2023 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Recombinant NiV M engages human importin alpha subunits 1 and 3 and undergoes nuclear import. Co-immunoprecipitation assays were preformed examining the interaction between NiV M NLS1 mutants (K84A, K84E, and R83A/K84A/K85A) (A–C) or NiV M NLS2 mutants (R256A, R257A, K258A, R256A/R257A, and R256A/R257A/K258A) (D–F). Assays were performed be immunoprecipitating HA-tagged IMPα1 (A,D), IMPα3 (B,E), and IMPα5 (C,F) isoforms and were probed for the presence of Flag-tagged NiV M. Expression of β-tubulin is used as a loading control for the input cell extracts.