Table 1.

Summary of the pathological nsSNPs and their effects.

Purpose	Tool Used	ANGPTL3		ANGPTL8
Effect of SNP on protein	nsSNP analyzer	Pathological	Neutral	Pathological	Neutral
		83	135	12	71
	SNP&GO	115	103	38	45
	PROVEAN	67	151	30	53
	PMUT	78	140	4	79
Total high-risk SNPs	Predicted by 3 or >3	67		12
Protein stability upon SNP	I-Mutant	Decrease	Increase	Decrease	Increase
		60	7	6	1
No. of conserved residues	ClustalO	50		All
Ligand binding	FTsite and COACH	8		0
Phosphorylation sites	Netphos 2.0	49		8
Glycosylation sites	YinOYang	8		5
Ubiquitination sites (≥2)	iUbiq-lys, CKSAAP UbSite, BDM-PUB	0		1
Palmitoylation sites	CSS-PALM 3.0	0		1
Methylation sites	PMes	7		0
Acetylation sites	PAIL and ASEB	15		3
Sumoylation sites	SUMOplotTM SUMOhydro and SUMOsp	3		0
Total PTM sites	-	82		18