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. 2023 Jun 1;21(6):340. doi: 10.3390/md21060340

Table 4.

Examples of carotenoid content in marine microalgae.

Species Carotenoids Collection Sites Experimental Conditions Quantification/Identification Methodologies Contents References
Chlorella vulgaris 211/52 β-carotene, lutein Grüental campus of the Zurich University of Applied Sciences, Wädenswil, Switzerland Open thin-layer bioreactor HPLC 50 μg/g β-carotene, 90 μg/g lutein, DW [201]
Dunaliella sp. FACHB-558 β-carotene Institute of Hydrobiology, Chinese Academy of Sciences, China Two-step cultivation in anaerobically digested poultry litter wastewater Spectrophotometry 7.26 mg/L, FW [193]
Isochrysis zhangjiangensis Fucoxanthin Chinese Academy of Sciences, China Photo-autotrophically cultured at 23 °C in a bubble column photobioreactors, supplemented with f/2 medium (nutrient, trace metal, and vitamin solutions) HPLC 23 μg/g, DW [202]
Haematococcus plivualis Astaxanthin Umeå University, Sweden First step: inoculum in Bold’s basal medium, OD750 of 0.1, 20 °C, cool white LED, 1 L/min of air, 7 days. Second step: grown in a multi-cultivator with cool white LED HPLC 19.1 mg/g, DW [203]
Desmodesmus sp. Lutein and zeaxanthin Isolated from a wastewater treatment system, Kalundborg Kommune, Copenhagen, Denmark Industrial wastewater in Schott bottles, stirred, aerated (2% CO2), and with fluorescent lights (200 µmol photon/m2·s) HPLC 5.11 mg/g lutein and 0.28 mg/g zeaxanthin, DW [204]
Nannochloropsis salina 40.85 β-carotene and violaxanthin Algae culture collection (SAG), University of Gottingen, Germany Industrial wastewater in Schott bottles, stirred, aerated (2% CO2), and with fluorescent lights (200 µmol photon/m2·s) HPLC 2.22 mg/g β-carotene and 1.68 mg/g violaxanthin, DW [204]
Phaeodactylum tricornutum Diadinoxanthin and diatoxanthin - Industrial wastewater in Schott bottles, stirred, aerated (2% CO2), and with fluorescent lights (300 µmol photon/m2·s) HPLC 2.17 μg/g diadinoxanthin and 1.55 μg/g diatoxanthin, DW [204]
Chlorella C.S1 β-carotene and lutein - Industrial wastewater in flat panel reactors with fluorescent lights (2000 µmol photon/m2·s) HPLC 1.4 mg/g β-carotene and 3.22 mg/g lutein, DW [204]
Nannochloropsis limnetica 18.99 Neoxanthin and violaxanthin Algal culture collection (SAG), University of Gottingen, Germany Industrial wastewater in Schott bottles, stirred, aerated (2% CO2), and with fluorescent lights (200 µmol photon/m2·s) HPLC 0.42 mg/g neoxanthin and 1.22 mg/g violaxanthin, DW [204]
Chlorococcum sp. Lutein Umeå University, Sweden Multi-cultivator with high light/cold stress in BG11 HPLC 15.5 mg/g, DW [205]
Scenedesmus sp. Lutein Umeå University, Sweden Multi-cultivator with high light/cold stress in BG11 HPLC 10.7 mg/g, DW [205]

DW, dry weight; HPLC, high-performance liquid chromatography.