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. Author manuscript; available in PMC: 2023 Jun 28.
Published in final edited form as: Cell Rep. 2023 May 10;42(5):112494. doi: 10.1016/j.celrep.2023.112494

KEY RESOURCES TABLE.

REAGENT or RESOURCE SOURCE IDENTIFIER
Antibodies
Rabbit Anti-mouse CRAMP Nizet et al., 20214 N/A
Goat anti-rabbit IgG(Fab)2-AlexaFluor488 Invitrogen Cat#: A11070, Lot#: 1040038, RRID: AB_2532697
Bacterial and virus strains
Staphylococcus hominis A9 Nakatsuji et al., 201723 N/A
Staphylococcus hominis A9 ΔLanti Nakatsuji et al., 201728 N/A
Staphylococcus epidermidis G9 Nakatsuji et al., 201723 N/A
Staphylococcus epidermidis D1 Nakatsuji et al., 201723 N/A
Staphylococcus hominis C4 Nakatsuji et al., 201723 N/A
Staphylococcus hominis C5 Nakatsuji et al., 201723 N/A
Staphylococcus hominis PR-A3 Nakatsuji et al., 201723 N/A
Staphylococcus epidermidis N009-G7 Nakatsuji et al., 201723 N/A
Staphylococcus epidermidis A11 Nakatsuji et al., 201723 N/A
Staphylococcus hominis C2 Nakatsuji et al., 201723 N/A
Staphylococcus hominis AMT2-A12 Nakatsuji et al., 201723 N/A
Staphylococcus hominis AMT4-D12 Nakatsuji et al., 201723 N/A
Staphylococcus aureus ATCC35556 ATCC Cat#: ATCC35556
Chemicals, peptides, and recombinant proteins
IMAGE-IT FX signal enhancer Invitrogen Cat#: I36933, Lot#: 2020111
5% Imiquimod cream Perrigo Cat#: NDC45802-368-00, Lot#: 129235
Contril cream for Imiquimod VWR Cat#: 56614-414, Lot#: N/A
MC903 TOCRIS Cat#: 2700, Batch#: 6A/248251
0.1% Triamcinolone Perrigo Cat#: NDC45802-055-35
Vaseline (vehicle control for triamcinolone) Uniliver Cat#: 67352423
Hanks’ balanced salt solution Thermo Fisher Scientific Cat#: 14175095
Abtibiotic-antimycotic cocktail Thermo Fisher Scientific Cat#: 15240062
deoxyribonuclease I Millipore Sigma Cat#: 4716728001
Liberase TL Millipore Sigma Cat#: 05401020001, Lot#: 56708200
HEPES solution Thermo Fisher Scientific Cat#: 15630080
Sodium pyruvate Thermo Fisher Scientific Cat#: 11360070
collagenase type IV Millipore Sigma Cat#: 11088882001, Lot#:
RNA later Invitrogen Cat#: AM7021, Lot#: 01085002
Fetab Bobine Serum GEMINI Bio Products Cat#: 900-108, Lot#: A08G001
Ovalubumin MP Biomedicals Cat#: 950512, Lot#: 8711K
Tryptic soy broth Millipore Sigma Cat#: T8907-1KG, used multiple lots
Bacto agar BD Cat#: 214010, Lot#: 1229826, used multiple lots
Bair-Parker agar BD Cat#: 276840, Lot#: 1272665
Egg yolk tellurite BD Cat#: 212357, Lot#: 2019838
Mannitol salt agar BD Cat#: 211407, Lot#: 0315698
Egg yolk emulsion HiMedia Cat#: FD045-100MLX1VL, Lot#: 0000530825, used multiple lots
RPMI media GIBCO Cat#: 11875-093, Lot#: 2436322
TE buffer Invitrogen Cat#: 12090-015, Lot#: 1691859
Tween 20 ThermoFisher Cat#: 28320, Lot#: VJ307818
Triton X-100 ThermoFisher Cat#: 28314, Lot#: VJ308263
LL-37 Genemed Synthesis, Inc. Custom Peptide
human beta-defensin 2 Peptide International Cat#: ODF-4338-s, Lot#: 671212
human beta-defensin 3 Peptide International Cat#: PDF-4382-s, Lot#: 660912
CRAMP (GLL-33) Genemed Synthesis, Inc. Custom Peptide
LongAmp Hot Start Taq 2X Master Mix New England BioLabs Cat#: M0533S
Agencourt AMPure XP beads Beckman Coulter Cat#: A63881
Critical commercial assays
PureLink Microbiome DNA extraction kit Invitrogen Cat#: A29789, Lot# 2077129
PureLink RNA extraction kit Invitrogen Cat#: 12183025, Lot#: 2346694
MACS dead cell removal kit Miltenyi Biotec Cat#: 130-090-101, Lot#:5210411028
PCR Barcoding Kit Oxford Nanopore Technologies Cat#: SQK- PBK004
KAPA2G Robust HotStart ReadyMix PCR Kit KAPA Biosystems Cat#: KK5701
Deposited data
Single Cell RNA-seq data This paper DDBJ sequence read archive (www.ddbj.nig.ac.jp), accession number: DRA015287
Experimental models: Organisms/strains
Wild-type Balb/c mice Jackson Laboratory Also known as Balb/cj, Strain #:000651, RRID:IMSR_JAX:000651
Camp−/− Balb/c mice Nizet et al., 20014 N/A
Flgft/ft Balb/c mice Gifted by Dr. Raif Geha (Nakatsuji et al., 201618) N/A
Il4ra−/− Balb/c mice Jackson Laboratory Also known as BALB/c-Il4ratm1Sz/J, Strain #:003514, RRID:IMSR_JAX:003514
Oligonucleotides
Primers for Il4 qPCR Integrated DNA technologies Mm.PT.58.7882098
Primers for Il13 qPCR Integrated DNA technologies Mm.PT.58.31366752
Primers for Il17a qPCR Integrated DNA technologies Mm.PT.58.6531092
Primers for Tslp qPCR Integrated DNA technologies Mm.PT.58.41321689
Primers/Probe for Camp qPCR Applied Biosystems Forward: CTTCACCAGCCCGTCCTTC, Reverse: CCAGGACGACACAGCAGTCA, Probe: FAM-CAGAGGATTGTGACTTCA-MGB
Primers for Defb4 qPCR Integrated DNA technologies Mm.PT.58.29993789
Primers for Defb14 qPCR Integrated DNA technologies Mm.PT.58.41499310
Primers/Probe for Gapdh qPCR Applied Biosystems Forward: CTTAGCACCCCTGGCCAAG, Reverse: TGGTCATGAGTCCTTCCACG, Probe: VIC-CATCCATGACAACTTTGGTA-MGB
Primers for ShA9-lantibiotic-alpha qPCR Integrated DNA technologies Forward: GAGGAGCTACACCGACTATTAC, Reverse: CACATCTAGAAGAGCAAGCTAATG
Software and algorithms
GraphPad Prism GraphPad Version 9.4.1
Other
Swab Puritan 25-1506 1PF BT BL
Tegaderm wound dressing film 3M Cat#: 1620