Figure 11.

(A) (i) TEM image of CTC membrane-coated Fe₃O₄@SiO₂ NPs (scale bar: 100 nm). (ii) Microscopic images of cells captured from mimicked clinical blood samples and identified with three-color immunocytochemistry. The merged image shows the overlapping of nuclei (Hoechst), CD45-FITC, and NIR nanobioprobe-cultured cells (scale bar: 50 µm). Reproduced with permission from [183], copyright Wiley-VCH 2020. (B) (i,ii) represent fluorescence micrographs and corresponding intensity analysis of different breast cancer subtypes treated with 50 µM of ZGM231 (MDA-MB-231 cell membrane-coated) and ZGMM (MCF-7 cell membrane-coated), respectively (scale bar: 50 μm). (iii) Whole-body fluorescence images of tumor-bearing mice injected with ZGM231 (60 mg/kg) and ZG (coating-free, 60 mg/kg) for 4 days. The white arrows marked the position of tumors (right flank: tumor, left flank: MCF-7 tumor). (iv) Quantification of the fluorescence signal after whole-body imaging (*** p < 0.001). Reproduced with permission from [185], copyright Wiley-VCH 2021.