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. Author manuscript; available in PMC: 2024 Apr 24.
Published in final edited form as: Dev Cell. 2023 Apr 12;58(8):645–659.e4. doi: 10.1016/j.devcel.2023.03.008

Figure 4: Dpp homodimers can only be detected in the absence of Gbb. A.

Figure 4:

Scheme of the experiment in A’. HATrap is expressed in posterior cells using the hh-Gal4 driver in a background containing both HA:Dpp and OLLAS:Dpp. If homodimers exist, HA trapping should lead to OLLAS:Dpp accumulation. A’. Expression of HATrap in posterior cells does not lead to accumulation of OLLAS:Dpp (right panel) compared to control (left panel). A’’ Quantifications of extracellular OLLAS:Dpp in the absence and presence of HATrap (control n=5; HA-Trap n=7). B. Scheme of the experimental setup of B’. HATrap is expressed in the posterior compartment using hh-LexA while gbbRNAi is expressed in the anterior stripe using ptc-Gal4, in a HA:dpp, OLLAS:dpp background. B’. Expression of gbbRNAi leads to the indirect accumulation of OLLAS:Dpp when trapping HA:Dpp (right panel) compared to no gbbRNAi expression. B’’. Quantifications of extracellular OLLAS:Dpp of the panels in B’ (control n=6; HA-Trap n=7). Scale bars 50 μm.