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. 2023 May 31;15(6):1305. doi: 10.3390/v15061305

Figure 1.

Figure 1

Experimental Design. (A): Protocol 1: Impact of age on immune responses: eight groups of ten 4–6-week-old CR were infected, (IN), with RSV (RSV primed). After 8 weeks, four groups were immunized with UC-3 F/F+H/G VLPs, or DS Cav1 F/F +H/G VLPs, or infected a second time with RSV (IN), or mock immunized (young). After another 8 weeks, these animals were challenged with RSV and sacrificed four days later. A second cohort of four groups of ten animals were similarly immunized at 24 weeks after the RSV prime (elderly). Eight weeks after immunization, these animals were challenged with RSV, (IN), and sacrificed four days later. (B): Protocol 2: Impact of two immunizations on immune responses: forty 4–6-week old CR were divided into four groups of ten animals. All animals were infected with RSV (RSV prime, day 0). Group 1 CR were immunized with UC-3 F/F+H/G VLPs on day 56 and mock immunized on day 196. Group 2 CR were mock immunized on day 56 and immunized with UC-3 F/F+H/G VLPs on day 196. Group 3 CR were immunized with UC-3 F/F+H/G VLPs on both days 56 and 196. Group 4 CR were mock immunized on day 56 and day 196. All animals were challenged with RSV on day 252 and sacrificed on day 256.