Figure 3.

Anti-IgG titers in animals in protocol 2. The sera from females and from males in each group at each time point were pooled separately. The ng/mL of anti-pre-F IgG in the sera pools from all four groups of males and females at each time point was measured by ELISA with soluble UC-3 F protein as target (A–D) or soluble DS-Cav1 F as target (E–H). The ng/mL of anti-G IgG was measured by ELISA using soluble G protein as the target (I–L). Each serum pool was assessed three times and the results of all three assays are shown with the averages indicated by a black line. Closed symbols, females; open symbols, males. Statistical analyses of differences between groups and sex differences were accomplished by one-way ANOVA, with Sidak’s multiple comparisons test. * p < 0.02; ** p < 0.005; *** p < 0.0005; **** p < 0.0001. ns: non-significant.