Figure 5.

The role of rho kinases in actin fiber contraction, actin depolymerization, and filament stabilization within a TM/SC or CM cell is shown here in a schematic format. The blockage of such processes and events by rho kinase (ROCK) inhibitors such as ripasudil and netarsudil causes the eventual relaxation of the cells. The inset with one yellow star demonstrates the enhancement of fluid outflow from the TM induced by the ROCK inhibitor fasudil in perfused anterior segments of porcine eyes ex vivo. The inset with two yellow stars illustrates the effect of ROCK inhibitor Y-27632 alone or on endothelin-1 (ET-1)-induced contraction of bovine TM cells under in vitro culture conditions, and the changes in F-actin, phosphorylated myosin light chain (ppMLC), and vinculin induced by these compounds.