Table 1.

Steps of the sterilization process.

Groups	Intervention	Time
1	Fat and muscle scrapping and 0.9% saline wash (Natural state)	N/A
2	2 × 2% Chlorhexidine baths + Solution: 50% Glycerol–49% Saline (0.9%) 1% PMF (Penicillin + Metronidazole + Fluconazole solution) +	2 × 30 min 24 h at 4 °C
3	Solution: 75% Glycerol–24% Saline (0.9%) 1% PMF +	3 h at 37 °C
4	Solution: 99% Glycerol–1% PMF	3 h at 37 °C