Figure 5.

Nonsense mutants as a product of NTG and 5-fluorouracil (FLU) mutagenesis of φ6h1s. The nonsense mutant selection and assignment to the different mutant’s classes based on missing proteins. (A) Autoradiogram of the protein synthesis in rifampin treated infected nonpermissive host cells. The samples were radiolabeled with ¹⁴C—leucine. The protein patterns are labeled a to k. Lane a was the control, noninfected samples, b was the cells infected with φ6h1s. The other patterns were from the bacteriophage mutants. (B) Nonsense mutant classes based on the missing proteins. “Figure 1” quote in subfigure B indicates Figure 5, subfigure A. The image and table reprintedd from Ref. [34] Sinclair J.F. et al., 1976, copyright BMC.