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. 2023 Jun 17;12(12):2353. doi: 10.3390/plants12122353

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© 2023 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Isozymatic NADP-ME activity in green sweet pepper fruits in an enriched 50–75% ammonium–sulfate protein fraction. Protein samples (240 µg per lane) were separated by non-denaturing polyacrylamide gel electrophoresis (PAGE; 8% acrylamide), and activity was detected using the NBT method (left). NADP-ME isozymes were labeled I–IV. Densitometric analysis of these isozymes and their relative quantification (%) were conducted using the ImageJ program according to the isozyme profiles obtained (right).