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. 2023 Jun 27;30:45. doi: 10.1186/s12929-023-00927-1

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 3 — FEACR inhibits H/R-induced ferroptosis in cardiomyocytes. a The expression of FEACR was inhibited in cardiomyocytes measured by qPCR. b CCK-8 kit determines the cell survival rate after FEACR inhibition. c The expression of ferroptosis-related proteins after the knockdown of FEACR was revealed by Western blot. d The content of lipid peroxidation production MDA in cardiomyocytes upon FEACR inhibition. e, f Cell survival rate and MDA content were measured after overexpression of FEACR in cardiomyocytes subjected to H/R treatment. g QPCR analysis measured the ptgs2 level after overexpression of FEACR in H/R-treated cardiomyocytes. h Iron content was determined by ferrous iron kit in cardiomyocytes. i Quantitative statistics of ROS content stained with C11-BODIPY in cardiomyocytes. a n = 3 biological replicates; b n = 5 biological replicates; c n = 3 biological replicates; d, f–h n = 5 biological replicates; e n = 6 biological replicates; i n = 6 biological replicates. Data were mean ± SD, and the P-value was calculated by One-way ANOVA. The experiment technically repeats three times