TABLE 3.
Bacterial strains and plasmids used in this work
| Strain or plasmid | Genotype and/or relevant characteristics | Source and/or reference |
|---|---|---|
| Strains | ||
| Stenotrophomonas maltophilia | ||
| K279a (wild type) | Clinical isolate and the genetic reference strain | Laboratory collection, 58 |
| K279aΔsmlt2053 | K279a carrying a deletion in gene Smlt2053 | This study |
| K279aΔsmlt2053-2051 | K279a carrying a deletion in operon Smlt2053-Smlt2051 | This study |
| Xanthomonas campestris pv. campestris | ||
| 8523 pL6engGUS | rpfF mutant, plasmid pLAFR6 carrying engXCA:gusA fusion. DSF reporter strain | 59 |
| Escherichia coli | ||
| DH5α | F− Φ80lacZΔM15 Δ(lacZYA-argF) U169 recA1 endA1 hsdR17(rK− mK+) phoA supE44 thi-1 gyrA96 relA1λ− | Laboratory collection, 70 |
| SY327 | Δ(lac pro) argE(Am) recA56 rif r nalA λ pir | 71 |
| BL21(DE3) | F– ompT gal dcm lon hsdSB(rB– mB–) λ(DE3 [lacI lacUV5-T7p07 ind1 sam7 nin5]) [malB+]K-12(λs) | Novagen |
| Plasmids | ||
| pGPI-SceI-XCm | Mobilizable suicide vector; carries the R6Kγ origin of replication, the I-SceI recognition site, and a xylE reporter gene, Cmr, Tpr | 67 |
| pΔsmlt2053-US’DS’ | pGPI-SceI-XCm containing the upstream and downstream flanking DNA regions of the smlt2053 gene | This study |
| pΔsmlt2053-2051-US’DS’ | pGPI-SceI-XCm containing the upstream and downstream flanking DNA regions of the smlt2053-smlt2051 operon | This study |
| pRK2013 | RK2-derived helper plasmid carrying the tra and mob genes for mobilization of plasmids containing oriT, Kanr | 72 |
| pDAI-SceI-SacB | Mobilizable broad host range plasmid; carries the gene for the I-SceI homing endonuclease and the sacB gene, Tetr | 67, 73 |
| pBBR1MCS-1 | Broad-host-range cloning vector used for complementation, low copy, Cmr | 74 |
| pBBR1MCS-1-smlt2053 | pBBR1MCS-1 with the gene Smlt2053 inserted between sites XbaI and HindIII, Cmr | This study |
| pET28a-TEV | Bacterial expression vector with T7-lacO promoter, hexa His tag, TEV cleavage site, Kanr | 75 |
| pET28a-Smlt2053 | Plasmid for Smlt2053 protein production in E. coli | This study |