TABLE 2.

The PCR primers used in this study to amplify the CS7, CS12, CS14, CS17, and CS21 major subunit genes, with restriction site NcoI underlined in each forward primer and EagI in the reverse primer

Primer	Sequences (5′ → 3′)
CS7(CsvA)-F	CGCCATGGAAATGGCAACCGTATCAG
CS7(CsvA)-R	TCGGCCGCTTTACGCCACATCCGAAGCACTT
CS12(CswA)-F	CATGCCATGGAAATGGCTGTTACTCTAATGAATAGCTCA
CS12(CswA)-R	GAGTCGGCCGCTTTATGAACACCAACAGTATTAAGTGAA
CS14(CsuA)-F	CATGCCATGGAA ATGGCTCTGAGCACAATATTTGTAG
CS14(CsuA)-R	GAGTCGGCCGCTTTAGTTTGTCGATTGAGTCATTACAATGGA
CS17(CsbA)-F	CTAGCTAGCTAGATGGCTATGGCGACTCTGTTTGCCA
CS17(CsbA)-R	GAGTCGGCCGCTTTATAATACCTTGGTAATTGCCTGCCTCA
CS21(LngA)-F	CATGCCATGGGCATGAGCCTGCTGGAAGTTATCATTGTT
CS21(LngA)-R	GAGTCGGCCGCTTTAACGGCTACCTAAAGTAATTGAG