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. 2023 Jun 28;5(3):zcad032. doi: 10.1093/narcan/zcad032

Figure 3.

Figure 3.

Hypoxic expression of PFKFB3 requires HIF-1α and PKM2. (A) ChIP assay of HIF-1α and PKM2 performed in WT BBS and BBS Mut MCF7 cells. (B) ChIP assay of HIF-1α and PKM2 in WT BBS, BBS Mut HCC1806 and HIAKO cells. (C) ChIP assay of p300 and H3K9Ac in WT BBS and BBS Mut MCF7 cells under hypoxia. Luciferase assay performed in WT BBS and BBS Mut cell lines of (D) MCF7 and (E) HCC1806 shows the reduction in the FLuc activity of HRE1 and HRE2 only in the WT BBS cell lines upon performing HIF-1α knockdown. Immunoblot analysis demonstrating the effect of HIF-1α knockdown on PFKFB3 expression in WT BBS and BBS Mut cell lines of (F) MCF7 and (G) HCC1806. For figures (F), and (G) representative images are provided. Error bars show mean values ± SD (n = 3 unless otherwise specified). As calculated using two-tailed Student's t-test, *P ≤ 0.05, **P ≤ 0.01, ***P ≤ 0.001, ****P ≤ 0.0001.