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. 2023 Jun 28;5(3):zcad032. doi: 10.1093/narcan/zcad032

Figure 5.

Figure 5.

Shikonin treatment affects PFKFB3 expression in hypoxic breast cancer cells. Immunofluorescence imaging analysis and quantification showing blocked PKM2 nuclear translocation upon shikonin treatment in WT BBS (A) MCF7 and (B) HCC1806 cells. Magnification used: 60×. Immunoblot analysis of PFKFB3 expression upon treating WT BBS and BBS Mut cell lines of (C) MCF7 and (D) HCC1806 with shikonin for 24 h under hypoxia. Luciferase assay performed upon subjecting WT BBS and BBS Mut cells of (E) MCF7 and (F) HCC1806 to shikonin treatment under hypoxia. (G) PFK assay performed in WT BBS MCF7 and HCC1806 cells showing the reduced PFK activity upon shikonin treatment in hypoxia. For figures (A), (B), (C), (D) and (G), representative images are provided. Error bars show mean values ± SD (n = 3 unless otherwise specified). As calculated using two-tailed Student's t-test, *P ≤ 0.05, **P ≤ 0.01, ***P ≤ 0.001, ****P ≤ 0.0001.