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. 2023 May 28;59(6):1044. doi: 10.3390/medicina59061044

Figure 2.

Figure 2

Western blot analysis of GRP78, NFkB and SREBP1c in the rat liver tissue. (A) GRP78, NFkB and SREBP1c protein bands in the liver tissue of the control group after 8 weeks (1) and 16 weeks (2); the restriction group after 8 weeks (3) and 16 weeks (4); and the treatment group after 8 weeks (5) and 16 weeks (6). The control group was given a normal diet without HSE, the restriction group was given a vitamin B12-restricted diet without HSE, and the treatment group was given a vitamin B12-restricted diet and HSE at 400 mg/kg weight/day. GADPH was used as an internal control. (B) GRP78-relative protein quantification analysis; (C) NFkB analysis relative to protein quantification; and (D) SREBP1c-relative protein quantification analysis. Bars represent different treatment ages for each group of experimental animals. HSE = Hibiscus sabdariffa ethanol extract, GRP78 = glucose-regulated protein-78, NFkB = nuclear factor kappa B, SREBP-1c = sterol regulatory element-binding protein 1c, GADPH = glyceraldehyde 3-phosphate dehydrogenase. Black box: control group; white box: Vit.B12 restriction group; grey box: Vit B12 restriction + HSE. Data are presented as the mean ± SD. Mean differences were calculated by one-way ANOVA followed by Tukey’s post hoc test. The mean difference was significant between control group and restriction group (*a p < 0.05) and the treatment group compared to the restriction group (*b p < 0.05, **b p < 0.01).