TABLE 3.
Copper content of C. metallidurans mutant cellsa
| Bacterial strain |
1,000 Cu/cell |
Bacterial strain |
1,000 Cu/cell |
||
|---|---|---|---|---|---|
| Added Cu(II) (μM) | 0 | 25 | Added Cu(II) (μM) | 0 | 25 |
| Δcus | 7.59 ± 2.64 | 49.6 ± 12.9 | Δcus ΔgshA | 5.13 ± 0.15 | 36.3 ± 5.7 |
| AE104 | 5.97 ± 1.00 | 32.8 ± 5.6 | AE104 ΔgshA | 6.11 ± 1.41 | 23.0 ± 2.5 |
| Δgig Δcus | 7.33 ± 2.02 | 28.1 ± 3.0 | Δgig Δcus ΔgshA | 6.58 ± 0.74 | 25.3 ± 1.3 |
| Δgig | 7.01 ± 1.21 | 26.2 ± 2.7 | Δgig ΔgshA | 5.02 ± 0.47 | 24.4 ± 3.5 |
| Δcop Δgig | 5.67 ± 1.05 | 36.3 ± 7.5 | Δcop Δgig ΔgshA | 6.69 ± 0.41 | 253 ± 17 |
| Δcop | 7.83 ± 3.20 | 52.4 ± 8.4 | Δcop ΔgshA | 4.62 ± 0.67 | 125 ± 25 |
| Added Cu(II) (μM) | 0 | 1 | Added Cu(II) (μM) | 0 | 1 |
| AE104 | 6.88 ± 1.29 | 20.5 ± 3.9 | |||
| Δcop Δcus | 7.06 ± 1.33 | 26.0 ± 3.2 | Δcop Δcus ΔgshA | 5.00 ± 0.53 | 45.5 ± 20.3 |
| Δcop Δcus Δgig | 6.71 ± 2.21 | 30.6 ± 8.4 | Δcop Δcus Δgig ΔgshA | 4.71 ± 0.62 | 33.3 ± 12.4 |
| Δcup | 9.00 ± 3.41 | 19.9 ± 6.1 | Δcup ΔgshA | 5.50 ± 0.94 | 19.1 ± 2.6 |
| Δcup Δgig | 6.59 ± 2.29 | 20.6 ± 7.0 | Δcup Δgig ΔgshA | 6.02 ± 0.75 | 17.5 ± 0.8 |
| Δcup Δcus | 5.22 ± 1.16 | 20.3 ± 3.5 | Δcup Δcus ΔgshA | 5.09 ± 0.39 | 19.6 ± 1.1 |
| Added Cu(II) (μM) | 0 | 0.02 | Added Cu(II) (μM) | 0 | 0.02 |
| AE104 | 6.92 ± 2.10 | 10.8 ± 0.8 | |||
| Δcop Δcup | 6.48 ± 2.23 | 8.0 ± 1.0 | Δcop Δcup ΔgshA | 6.03 ± 0.58 | 13.6 ± 4.0 |
| Δcup Δcus Δgig | 6.11 ± 2.12 | 9.7 ± 0.3 | Δcup Δcus Δgig ΔgshA | 3.30 ± 1.03 | 8.0 ± 1.3 |
| Δcop Δcup Δcus | 8.04 ± 3.82 | 8.3 ± 3.2 | Δcop Δcup Δcus ΔgshA | 5.64 ± 0.44 | 9.8 ± 0.8 |
| Δcop Δcup Δcus Δgig | 6.32 ± 1.88 | 9.2 ± 0.3 | Δcop Δcup Δcus Δgig ΔgshA | 5.78 ± 1.10 | 12.0 ± 1.4 |
| Δcop Δcup Δgig | 5.85 ± 1.03 | 7.4 ± 2.6 | Δcop Δcup Δgig ΔgshA | 3.68 ± 0.67 | 11.5 ± 1.2 |
a
The cells were cultivated in medium without (negative control) or with copper at the indicated concentrations. The copper content of the Tris-buffered mineral salts medium without added copper was 38 ± 23 nM (n = 4), as determined by ICP-MS. Cells were mineralized in 67% nitric acid at 70°C for 2 h. Samples were diluted to a final concentration of 2% nitric acid. Bold indicates ([Q ≥ 1.5 OR Q ≤ 0.66] AND D > 1) in the comparison to the parent strain AE104 cultivated in the presence of the same copper concentration.