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. 2023 May 16;89(6):e00220-23. doi: 10.1128/aem.00220-23

TABLE 2.

Determination of specific enzyme activities of wild-type LsNit, RsNit, and SmNit and mutants

Enzyme activity toward substrates (U · mg−1)
1 2 3 4 5 6 7 8
Nitrilase graphic file with name aem.00220-23-f010.jpg graphic file with name aem.00220-23-f011.jpg graphic file with name aem.00220-23-f012.jpg graphic file with name aem.00220-23-f013.jpg graphic file with name aem.00220-23-f014.jpg graphic file with name aem.00220-23-f015.jpg graphic file with name aem.00220-23-f016.jpg graphic file with name aem.00220-23-f017.jpg
LsNit 24.69 5.80 7.06 7.88 64.47 24.09 159.73 26.70
Ls-W165G-V193L 164.02 50.87 54.42 9.01 16.57 11.40 12.73 7.38
RsNit 9.97 1.02 0.27 0.13 0.22 0.0065 0.00075 0.00016
Rs-W164G-V192L 64.29 21.13 8.66 0.49 0.049 0.00085 0.000027 0
SmNit 1.91 0 0 0 1.88 0 5.75 0
Sm-W184G-V212L 7.14 1.23 0.88 0 0.80 0 0.91 0