Figure 1.

Artificially induced decidualization leads to the upregulation of SHP2 in hESCs. (A) Real-time PCR analysis of the transcriptional levels of SHP2, IGFBP1, and PRL in hESCs treated with MPA and db-cAMP for 4 days. Error bars denote the s.d. (n = 3). Data were calibrated to the most highly expressing sample in hESCs (red color). (B) Immunoblotting of the protein level of SHP2 in hESCs treated with MPA and db-cAMP for 4 days. Bar figures represent the ratios of densities (SHP2/B-actin). Error bars denote the s.d. (n = 3). (C) Real-time PCR analysis of the transcriptional levels of SHP2 in ESCs and DSCs. Error bars denote the s.d. (n = 10). Data were calibrated to the most highly expressing sample in ESCs (red color). (D) Immunoblotting of the protein level of SHP2 in ESCs and DSCs. Bar figures represent the ratios of densities (SHP2/B-actin). Error bars denote the s.d. (n = 5). (E) Bar graph of SHP2 expression across human tissues. Red box indicates SHP2 expression in female reproductive tissues. Data were obtained from the Human Protein Atlas. ^***P < 0.001.