FIG 1.

(A) Isolation of suppressor mutants from a L. cremoris PSP-negative strain on solid GM17 medium. Larger colonies (indicated by white arrows) arise spontaneously among the small colonies formed by VES5748 (wpsH) PSP-negative strain. (B and C) Growth in liquid GM17 medium of MG1363, wpsH mutant, and its pbp2b derivatives (B) and of NZ9000, wpsA mutant, and its pbp2b derivatives (C). Each point represents the mean value of three biological replicates. The standard deviations for each value were very low and could not be clearly represented on the corresponding curves. (D) Generation time (G) determined in the exponential growth phase for each strain.