TABLE 3.
Primers used in this study
| Primer | Sequence (5′–3′)a |
|---|---|
| alpF1 | ATTGTGTCAATGGGAAGTGG |
| recR2 | GAGCAAAAGCGTAAATCTCG |
| pbp2b3s | TCAGGTAGCGAATAAGCTAG |
| pbp2b5 | AAAAGGAAAACGTGGGGATG |
| pbp2b6 | CAATTGGCAGTTTATGCTGC |
| pbp2b7 | TTTGTTCCGCCGTAAAGTTC |
| Pbp2B 1 pGh9 L | AGCTTGATATCGAATTCCTGCAGCCCTTGTCCTCATTGTGATGGTC |
| Pbp2B 2 | ACATTTGGACTCCAAGAATGAG |
| Pbp2B 3 | CTCATTCTTGGAGTCCAAATGTAAAAGCCCTCTTGAATGAGG |
| Pbp2B 4 pGh9 R | CGGCCGCTCTAGAACTAGTGGATCCCCCTCTCCACGGCTTTTGATAAG |
| pGH9L | ATGTTACAGTCTATCCCTGG |
| pGH9R | GTATACTACTGACAGCTTCC |
| Pbp2B del L | TTGGCGTGATTTAGGAATCC |
| Pbp2B del R | TTTACTGACTGCCTTGTCAG |
a
The overlaps between sequences to assemble pbp2b gene are shaded gray; the overlap between sequences to assemble amplified pbp2b gene into pGh9 are underlined.