FIG 1.

Schematic for CRISPR-based DNA and RNA detection in a one-pot reaction. When the TSV or WSSV target sequence is absent, there is no amplification and no CRISPR-Cas mediated cleavage of the reporter molecule. When the TSV or WSSV target sequence is present, there is isothermal amplification of the target sequence and simultaneous detection by CRISPR-Cas target binding and collateral cleavage of a fluorescent reporter. LAMP ± RT signifies the need for reverse transcriptase (RT) and LAMP for the amplification of the TSV RNA target or need for LAMP only for the amplification of the WSSV DNA target.