Figure 3.

Effects of deletion of VM1G_06867 on conidiation and conidia germination in Valsa mali. (A) Growth of pycnidia in wild type, VM1G_06867 single deletion mutant, complemented mutant, and double deletion mutant on PDA for 20 days PDA. (B) Quantification of produced pycnidia produced as described in Section 2. Data from three replicates are analyzed using the protected Fisher’s least significant difference (LSD) test. Different letters indicate statistically significant differences (Duncan’s new multiple range test, p < 0.05). (C) Coating of spore suspensions from different strains onto PDA media at 25 °C for 30 h. Bars = 10 μm.