Effects of VM1G_06867 on pathogenicity in Valsa mali. (A) Apple fruit is scalded and inoculated with mycelium agar plugs from the wild type, VM1G_06867 single deletion mutant, complemented mutant, and double deletion mutant. (B) The lesion length that is measured at 7 days post inoculation (dpi). The experiments are repeated three times. Different letters indicate statistically significant differences (Duncan’s new multiple range test, p < 0.05). (C) Apple twigs are scalded and inoculated with mycelium agar plugs from the wild type, VM1G_06867 single deletion mutant, complemented mutant, and double deletion mutant. Images are taken at 7 dpi. (D) The lesion length that is measured at 7 dpi. The experiments are repeated three times. Different letters indicate statistically significant differences (Duncan’s new multiple range test, p < 0.05).