Table 8.
Test of the West Nile virus set B primers with WNV-L2 homogenates.
| Viral Homogenate WNV L2—Sample Number | RT-PCR Ct Value | Host | Total Number of Trimmate Reads | Number of WNV Reads | % HCoverage | VCoverage | Consensus Sequence Length |
|---|---|---|---|---|---|---|---|
| 1 | 17 | Pica pica | 1134.961 | 295.649 | 100% | 3977.58 | 10.892 |
| 2 | 27 | Corvus cornix | 1448.962 | 284.947 | 100% | 3818.15 | 10.912 |
| 3 | 21 | Culex pipiens | 1733.553 | 385.088 | 100% | 5042.35 | 10.913 |
| 4 | 23 | Culex pipiens | 1528.111 | 339.207 | 100% | 4525.72 | 10.912 |
| 5 | 21 | Athene noctua | 1801.390 | 376.213 | 100% | 4946.53 | 10.878 |
| 6 | 22 | Culex pipiens | 1407.784 | 312.376 | 99% | 4201.89 | 10.879 |
| 7 | 19 | Passer domesticus | 1515.878 | 205.605 | 100% | 2770.45 | 10.914 |
| 8 | 30 | Corvus cornix | 1470.367 | 209.316 | 98% | 2799.57 | 10.872 |
| 9 | 30 | Pica pica | 2150.396 | 205.466 | 99% | 2662.61 | 10.868 |
| 10 | 27 | Sylvia atricapilla | 3649.208 | 281.102 | 99% | 3426.07 | 10.880 |
| 11 | 25 | Culex pipiens | 2094.249 | 349.710 | 100% | 4076.35 | 10.878 |
| 12 | 29 | Anopheles maculipennis | 3435.959 | 563176 | 100% | 5709.14 | 10.912 |
| 13 | 25 | Culex pipiens | 3120.601 | 281.102 | 100% | 4601.81 | 10.912 |
| 14 | 27 | Culex pipiens | 2193.040 | 259.483 | 97% | 3231.74 | 10.904 |
| 15 | 35 | Equus ferus caballus | 1623.442 | 133.088 | 93% | 1814.74 | 10.936 |