Table 9.

Test of the West Nile virus set A + B primers with WNV-L1 and WNV-L2 homogenates.

Viral Homogenate WNV L1	RT-PCR Ct Value	Host	Used Primers	Total Number of Trimmate Reads	Number of WNV Reads	% HCoverage	VCoverage	Consensus Sequence Length
1	L1 19	Corvus cornix	Set A	2347.380	351.292	99%	4177.69	10.963
			Set A + B	1118.615	287.672	99%	3987.12	10.963
2	L1 25	Larus michahellis	Set A	1813.567	129.834	88%	1805.95	10.952
			Set A + B	651.723	91.642	93%	1305.83	10.960
3	L1 18	Pica pica	Set A	2923.237	387.986	99%	4264.56	10.966
			Set A + B	4965.722	512.725	99%	4562.28	10.966
4	L1 28	Equus ferus caballus	Set A	1805.957	159.703	92%	2101.31	10.960
			Set A + B	2319.560	165.376	92%	2200.39	10.960
Viral Homogenate WNV L2	RT-PCR Ct Value	Host	Used Primers	Total Number of Trimmate Reads	Number of WNV Reads	% HCoverage	VCoverage	Consensus Sequence Length
1	L2 17	Pica pica	Set B	2059.659	351.670	100%	4232.78	10.892
			Set A + B	1134.961	295.649	100%	3977.58	10.892
2	L2 27	Corvus cornix	Set B	1793.046	187.826	100%	2156.81	10.926
			Set A + B	1448.962	284.947	100%	3818.15	10.912
3	L2 21	Culex pipiens	Set B	2219.785	312.851	100%	3515.05	10.926
			Set A + B	1733.553	385.088	100%	5042.35	10.913
4	L2 23	Culex pipiens	Set B	2045.957	275.354	100%	2943.77	10.926
			Set A + B	1528.111	339.207	100%	4525.72	10.912
5	L2 21	Athene noctua	Set B	3413.467	359.916	100%	4106.39	10.892
			Set A + B	1801.390	376.213	100%	4946.53	10.878
6	L2 22	Culex pipiens	Set B	3109.597	239.795	95%	2761.23	10.892
			Set A + B	1407.784	312.376	99%	4201.89	10.879
7	L2 19	Passer domesticus	Set B	1621.442	123.813	89%	1836.85	10.924
			Set A + B	1515.878	205.605	100%	2770.45	10.914