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Identification of the wheat–tetraploid Th. elongatum substitution line K17-1065-4 using the GISH and FISH techniques. The probes were (a,c,e) tetraploid Th. elongatum genomic DNA; (b,f) Oligo-pSc119.2 (green) and Oligo-pTa535 (red); (d) Chr6 (red). Arrows indicate chromosomes 6E. Scale bar: 10 lm.
