Figure 3.
Recombinant ghA, ghB, and ghC modules of human C1q block SARS-CoV-2 pseudoparticle entry into A549-hACE2+TMPRSS2 cells. SARS-CoV-2 pseudoparticles were pre-treated with ghA (A), ghB (B), or ghC (C) (3.33 × 102 μmol/mL) to determine if the recombinant modules of human C1q interfered with the ability of the pseudoparticles to enter the target cells. Luciferase reporter activity in A549-hACE2+TMPRSS2 cells that were transduced with pseudoparticles (and pre-treated with ghA, ghB, or ghC) was used. The background was subtracted from all data points. The data obtained were normalised with 0% luciferase activity defined as the mean of the relative luminescence units recorded from the control sample (A549-hACE2+TMPRSS2 cells + MBP + SARS-CoV-2 pseudoparticles). Data are shown as the normalized mean of three independent experiments carried out in triplicates ± SEM. Significance was determined using the two-way ANOVA test (*** p < 0.001) (n = 3).