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. 2023 May 29;15(6):1269. doi: 10.3390/v15061269

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© 2023 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Binding of C1q (A) or C4BP treated (B) SARS-CoV-2 pseudoparticles to A549-hACE2+TMPRSS2 cells. SARS-CoV-2 lentiviral pseudoparticles were used to transduce A549-hACE2+TMPRSS2 cells, which were pre-incubated with C1q or C4BP (40 µmol/mL). The wells were probed with rabbit anti-SARS-CoV-2 spike (1:200) polyclonal antibodies after being washed and fixed with 1% v/v paraformaldehyde for 1 min. The data obtained were normalised with 0% fluorescence defined as the mean of the relative fluorescence units recorded from the control sample (A549-hACE2+TMPRSS2 cells + SARS-CoV-2 lentiviral pseudoparticles). Three independent experiments were carried out in triplicates, and error bars are expressed as ± SEM. Significance was determined using the two-way ANOVA test (*** p < 0.001) (n = 3).