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. Author manuscript; available in PMC: 2023 Jun 28.
Published in final edited form as: Nat Metab. 2022 Jun 23;4(6):711–723. doi: 10.1038/s42255-022-00588-8

Extended Data Fig. 5 |. Effects of exogenous metabolites on ACC phosphorylation.

Extended Data Fig. 5 |

a, Immunoblot of total ACC and ACC serine 79 phosphorylation in HeLa cells cultured for 24 hours in media +lipids or −lipids without or with phenformin (100μM), pyruvate (1mM, Pyr), lactate (10mM, Lac), and/or acetate (200μM, Ac) as indicated. b, Immunoblot of FASN, phosphorylated PDHA (Serine 232, Serine 293, and Serine 300), total PHDA, PDK1, PDP1, and Vinculin in HeLa cells starved of lipids over time. c, Immunoblot of FASN, phosphorylated PDHA (Serine 293), total PDHA, and Vinculin in HeLa or H1299 cells overexpressing eGFP or constitutively mature SREBP1a. d, Immunoblot of phosphorylated PDHA (Serine 293) and Vinculin in HeLa cultured in −lipids for 24hrs, treated with vehicle, phenformin, antimycin, pyruvate, and/or alpha-ketobutyrate at the indicated doses.