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Binding of 7-Br-Trp. (a) Initial mF o − DF c density (green mesh, 3σ) before placing 7-Br-Trp. (b) Final 2mF o − DF c density (blue mesh, 1.5σ). The anomalous signal of bromine in 7-Br-Trp is shown as a yellow mesh (5σ). (c) 7-Br-Trp (blue C atoms) binds in the same pose as Trp (orange C atoms). (d) 5-Br-Trp (dark green C atoms) binds in a flipped orientation so that Br of 5-Br-Trp and 7-Br-Trp occupy the same space.
