Figure 4: kcc is a putative mature neuron marker.
(a) Subclustering of neurons expressing a glycine-gated chloride channel (dd_Smed_v4_11342_0_1) shows that smedwi-1 (dd_Smed_v4_659_0_1) and kcc (dd_Smed_v4_4389_0_1) are expressed in distinct clusters. This expression pattern is observed in all neuronal populations that express any of the ligand-gated chloride channel homologs. (b) Fractions of ligand-gated chloride channel expressing neurons that also express either smedwi-1 (blue) or kcc (magenta) at different time points in regeneration. Error bars: 95% confidence intervals calculated using a binomial distribution. p-values: significant (p<0.05, Welch’s t-test) changes compared to the previous time point. (c) A model for neural differentiation in the planarian nervous system.
