Figure 5.

Conditional knockout of CDK2 in adult mice confirms lack of a novel morphological change after CDK2 loss. A–C, Conditional knockout of CDK2 in adult mice using Cre-mediated recombination only causes a morphological change in the testis. A, Study outline. CDK2 cKO (genotype Cdk2^fl/fl; Rosa26^{tm(Cre/ERT2)+/−}) or WT (genotype Cdk2^fl/fl; Rosa26^{tm(Cre/ERT2−/−)} mice were administered 75 mg/kg tamoxifen by oral gavage once per day for 6 days and mice were analyzed 3 weeks or 24 weeks after tamoxifen administration. B, Summary of histopathology results 3 weeks after deletion of CDK2. H&E slides from at least 4 mice per gender were evaluated by a board-certified veterinary anatomic pathologist. C, Summary of histopathology results 24 weeks after deletion of CDK2. H&E slides from at least 8 mice per gender were evaluated by a board-certified veterinary anatomic pathologist. D and E, Treatment of CDK2 cKO mice with tamoxifen yields potent and long-lasting loss of CDK2 protein. D, Western blot for CDK2 in the bone marrow, ileum or testis 3 weeks after WT (“CDK2 cKO –”)or CDK2 cKO (“CDK2 cKO +”) mice have been administered 75 mg/kg tamoxifen for 6 days. E, Western blot for CDK2 in the bone marrow, ileum, or testis 24 weeks after WT or CDK2 cKO mice have been administered 75 mg/kg tamoxifen for 6 days. For each tissue, all samples were ran and transferred on the same gel/membrane but images were cropped to remove failed or unrelated samples. Molecular weight markers are in kDa.