Figure 6.

Homozygous CDK2^dTAG and CDK5^dTAG mice have reduced fecundity. A, Breeding of HOM CDK2^dTAG and CDK5^dTAG mice produces less pups than HET mice. Number of pups born per day from crossing heterozygotes (HET × HET, left) or homozygous (HOM × HOM, right) CDK2^dTAG or CDK5^dTAG mice. n = number of pups born. B, Breeding of HOM CDK2^dTAG or CDK5^dTAG mice produces less litters than HET or WT mice. Number of litters born per day from crossing heterozygotes (HET × HET), homozygous (HOM × HOM), or homozygous and wild-type (HOM × WT) CDK2^dTAG or CDK5^dTAG mice. n is number of litters born. C, Sperm from HOM CDK2^dTAG and CDK5^dTAG mice are less fertile than WT mice. For fertilization rates, oocytes from WT mice were inseminated with sperm from HOM WT, CDK2^dTAG or CDK5^dTAG mice and the next day the total number of 2 cell embryos was counted to determine the fertilization rate. For sperm morphological analyses, mice were euthanized and sperm was collected from the caudal epididymis and vas deferens, chilled, and analyzed on an IVOS machine. Liability is the percent of normal sperm in the sample. Data shown are 2 independent runs on the IVOS machine from 4 mice (2 mice per run). *** = p < .01, 2-way ANOVA with Dunnett’s multiple comparison test.