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. 2023 May 17;618(7967):1072–1077. doi: 10.1038/s41586-023-06191-5

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — a, Scheme of recombinant antibody screening. EV, extracellular vesicle; FACS, fluorescence-activated cell sorting. b, LDH released from Pam3CSK4-primed wild-type BMDMs after nigericin stimulation for 16 h in the presence of 1 μg ml⁻¹ of indicated antibody (dots represent different antibodies tested). Ninj1^−/−, Ninj1^−/− BMDMs; non-stim, non-stimulated wild-type BMDMs. The LDH score is the LDH release normalized against the no-antibody control. c, The percentage of YOYO-1⁺ NINJ1-expressing HEK293T cells when cultured with clone D1 or an isotype control antibody. Data are mean (circles) ± s.d. (shaded area) of three independent replicates. d, Flow cytometry histograms of propidium iodide-negative HEK293T cells surface-stained with anti-NINJ1 or anti-Flag antibodies. Cells are mock-transfected (light grey) or transfected with indicated NINJ1 constructs (dark grey). WT, wild type; ΔCT, NINJ1(Δ142–152); ΔNT, NINJ1(Δ2–73). In c,d, results are representative of three independent experiments.