Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2023 May 17;618(7967):1072–1077. doi: 10.1038/s41586-023-06191-5

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Extended Data Fig. 1 — a, Graphs indicate LDH release in cultures of wild-type BMDMs after priming, and then stimulation with nigericin for 16 h in the presence or absence (cont) of clone D1 Fab. Non-stim, non-stimulated control. Bars indicate the mean. Circles indicate 3 biological replicates. Results representative of 3 independent experiments. b, Graphs indicate the percentage of YOYO-1⁺ HEK293T NINJ1 expressing cells when cultured with or without (cont) clone D1 Fab. Circles are the mean ± s.d. (shaded area) of 3 independent replicates. Results are representative of 2 independent experiments. c, Flow cytometry histograms of propidium iodide-negative HEK293T cells surface-stained with anti-NINJ1 or anti-FLAG antibodies. Cells are mock transfected (light gray) or transfected with human NINJ1 (dark gray). Results representative of 3 independent experiments. d, Amino acid sequence alignment of human and mouse NINJ1. Predicted epitope of clone 25 (grey box) and clone D1 (blue box) are highlighted. e, Graph shows the release of DD-150 from Pam3CSK4-primed BMDMs in (a) after nigericin stimulation. Data are means (circles) ± s.d. (shaded area). Circles indicate 3 biological replicates (n = 3 animals). Results representative of 3 independent experiments. f, Graphs indicate cell viability (top) and LDH release (bottom) in BMDMs. Non-stim, non-stimulated control. Bars indicate the mean. Circles indicate 3 biological replicates (n = 3 animals). Results representative of 2 independent experiments.