Extended Data Fig. 6. Comparison of the PxxG switch and the HETY inactive motif between PCO371-bound and PTH-bound PTH1R.

(a) TMD region of the PCO371-bound PTH1R. The eye and square indicate views in (c–h). (b) Central polar networks of bound PTH-bound active PTH1R (left, violet), PCO371- PTH1R (center, green), and ePTH-bound inactive PTH1R (right, gray). (c–h) Superimposed structures of PTH-bound PTH1R (green), PCO371-PTH1R (violet), and ePTH-bound PTH1R (gray) are shown parallel to the membrane. Magnified views of the PYQ active motif and the PxxG active switch (c–d and f–g). Magnified views of the HETY inactive motif (e and h). (i) Superimposed structures of PCO371 (crimson) and ePTH-bound PTH1R.