Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2023 Jun 7;618(7967):1085–1093. doi: 10.1038/s41586-023-06169-3

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Extended Data Fig. 9 — (a) TMD structures of PCO371-bound PTH1R and six intracellular ligand-bound GPCRs are shown parallel to the membrane or from the intracellular side. Violet, PCO371-bound PTH1R; magenta, PCO371; orange, compound 2-bound GLP-1R; blue, compound 2, yellow, C-C motif chemokine receptor (CCR2)-RA-[R]-bound CCR2; violet, CCR2-RA-[R]; yellow-green, cmpd2105-bound CCR7; plum, cmpd2105; green, vercirnon-bound CCR6; brown, vercirnon; powder blue, cmpd-15PA-bound β₂AR; coral, cmpd-15PA; light cyan, cmpd-6FA-bound β₂AR; gold, cmpd-6FA. (b, c) Superimposed structures of compound2-bound GLP-1R, GLP-1-bound GLP-1R, LY3502970-bound GLP-1R, and inactive GLP-1R. White, GLP-1-bound GLP-1R; khaki, GLP-1; red, LY3502970 -bound GLP-1R; sky blue, LY3502970; gray, inactive GLP-1R. Compound2 binds with the intracellular portion of TM6, and compound2-bound GLP-1R exhibits the identical active conformation of TM6. (d) GloSensor cAMP responses in wild-type (WT) PTH1R and the TM6-replaced mutants upon endogenous agonists (PTH or glucagon) or PCO371 stimulation. Symbols and error bars represent mean and SEM, respectively, of three independent experiments with each performed in duplicate. (e) Cut-through view of PCO371–PTH1R–G_s and magnified view of the PCO371-G-protein interface. The PCO371-interacting Gα_s region is colored orange. (f) Sequence alignment of the C-terminal hook of Gα proteins.