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. 2023 Jun 15;24(7):1188–1199. doi: 10.1038/s41590-023-01528-8

Extended Data Fig. 3. Quantitative analysis of microglia surface area, soma size and density in different brain regions of WT and SALL1 EKO mice.

Extended Data Fig. 3

a,b. Representative brain section of the prefrontal cortex co-stained with IBA1 and DAPI. c,d,e. Prefrontal cortex - Quantification of surface area (n = 150 microglia/brain region/genotype), soma size (n = 124 microglia/brain region/geno- type), density (n = 142 microglia/brain region/genotype). f,g. Representative brain section of hippocampus co-stained with IBA1 and DAPI. h,i,j. Hippocampus - Quantification of surface area (n = 150 microglia/brain region/genotype), soma size (n = 124 microg- lia/brain region/genotype), density (n = 142 microglia/brain region/genotype). k,l. Representative brain section of striatum co-stained with IBA1 and DAPI. m,n,o. Striatum - Quantification of surface area (n = 150 microglia/brain region/genotype), soma size (n = 123 microg- lia/brain region/genotype), density (n = 150 microglia/brain region/genotype). p,q. Representative brain section of cerebellum co-stained with IBA1 and DAPI. r,s,t. Cerebellum - Quantification of surface area (n = 150 microglia/brain region/genotype), soma size (n = 123 microg- lia/brain region/genotype), density (n = 150 microglia/brain region/WT, n = 147 microglia/brain region/EKO). 15 ROIs, 4 sections per brain region, 2-3 mice/genotype. Unpaired two-tailed t-test with Welch’s correction was used to calculate significance. Data are represented as mean with s.d.