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. 2023 May 10;618(7967):1041–1048. doi: 10.1038/s41586-023-05974-0

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Extended Data Fig. 6 — a) Examples of CIP2A localization patterns in ruptured (γH2AX-positive) micronuclei of DLD-1 cells. Intensity measurements shown in Fig. 3a. Scale bar, 10 μm. b) Examples of CIP2A localization patterns in ruptured micronuclei in DLD-1 cells expressing cGAS-GFP and immunostained for cGAS accumulation (top) or the lack of acetylated H3K9 (bottom) in RPE-1 cells. Scale bar, 10 μm. c) Intensity measurements of distinct CIP2A localization patterns in micronuclei compared to the cytoplasm in the indicated cell lines. Box plot represents interquartile range with min-max; HeLa: none, n = 322, diffuse, n = 48, puncta, n = 30; RPTEC: none, n = 91, diffuse, n = 100, puncta, n = 69; RPE-1: none, n = 317, diffuse, n = 86, puncta, n = 34; RPE-1 H2AX^–/–: none, n = 274, diffuse, n = 47, puncta, n = 40 micronuclei pooled from 3-5 independent experiments. d) Frequency of CIP2A localization patterns in ruptured (γH2AX-positive, cGAS-positive, or H3K9ac-negative) micronuclei across a panel of human cell lines. Data represent mean; from left to right, n = 218, 99, 169, 134, 119, 76, 111, 211, 196, 92, 121, and 67 micronuclei pooled from 2 (RPE-1 H2AX^–/–) or 3 (all other conditions) independent experiments. e) Examples of TOPBP1 localization patterns in ruptured (γH2AX-positive) micronuclei of DLD-1 cells. Scale bar, 10 μm. f) Examples of TOPBP1 localization patterns in ruptured micronuclei in DLD-1 cells expressing cGAS-GFP and immunostained for cGAS accumulation (top) or the lack of acetylated H3K9 (bottom) in RPE-1 cells. Scale bar, 10 μm. g) Frequency of TOPBP1 localization patterns in ruptured (γH2AX-positive, cGAS-positive, or H3K9ac-negative) micronuclei across a panel of human cell lines. Data represent mean; from left to right, n = 323, 120, 172, 133, 143, 87, 160, 295, 221, 82, 230, and 88 micronuclei pooled from 3 independent experiments. For (d) and (g), DLD-1 cells were treated with DOX/IAA to induce Y chromosome micronuclei, HeLa and RPE-1 cells were treated with CENP-E/Mps1 inhibitors to induce random micronuclei, and RPTECs were transfected with Cas9 ribonucleoproteins targeting the chromosome 3p arm near the centromere to induce chromosome 3p micronuclei.

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