Extended Data Fig. 6. CD4+ effector T-cells cluster with antigen-presenting dendritic cells and macrophages at the invasive margin of a human melanoma.

Multiple iterative labeling by antibody neodeposition (MILAN) of a human melanoma obtained from (Pozniak, J., et al., 2022). An overview (a) over the whole tumour and a selected area of the tumour margin (b) are shown with multiple label combinations selected from the published panel. Insets (1-3) show exemplary sites of CD4+ T-cell juxtaposition with different myeloid subtypes expressing MHC-II, CD11c, and/or CD68. c, combinatorial overlays of different T-cell markers (white) with myeloid cell markers (red) in the insets (1-3).