Extended Data Fig. 8. Robust IFNγ-dependent eradication of established melanomas requires local adjuvant innate immune stimulation.
a, Experimental treatment protocol to address the impact of innate stimuli on myeloid cell activation and tumour control. b, Cell density (left) and representative contour plots quantifying the relative iNOS expression (right) in Ly6Chi mono-macs (NT vs CVTI *p = 0.0109) and neutrophils (NT vs CVTI *p = 0.0114, CVTI vs CVT *p = 0.0474) in tumours 5 days post-ACT, treated as indicated (mean ± SEM from n = 6-7 biologcally independent samples). c, Individual tumour growth curves in mice bearing established melanomas and treated as indicated. d, Experimental treatment protocol to address the impact of local innate stimuli on tumour control and (e) individual growth curves of mice bearing contralateral HCmel12 tumours, treated as indicated. f, Experimental treatment protocol to address the impact of IFNγ-blockade. g, Cell density (left) and relative iNOS expression (right) in Ly6Chi mono-macs and neutrophils in tumours 5 days post-ACT, treated as indicated (mean ± SEM from n = 6-7 biologically independent samples, *p = 0.0109). h, Individual tumour growth curves of mice bearing established melanomas and treated as indicated. Means between groups were statistically compared using a one-way ANOVA with Tukey post-hoc.